Register for more lightcycler 96 information last name first name email address institution or company street address city postal code country are you planning to purchase. The lightcycler 480 instrument ii is a rapid, platebased, thermal block cycler with integrated realtime, online detection capabilities. Jan 31, 20 lightcycler 96 realtime pcr system from roche life science duration. Lightcycler software roche, free lightcycler software roche software downloads, page 3. Silent and compact, this instrument will be the perfect benchtop companion for laboratory end users performing realtime pcr on a daily basis. Roche diagnostics corporation commercial education department 9115 hague road p. Used roche lightcycler 480 ii real time pcr system lab. Roche diagnostics lightcycler 480 plate 96 white lightcycler. This unit has a bad lamp housing the lightcycler 480 system is a highperformance, medium to highthroughput pcr platform that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array data validation. Roche diagnostics introduces ultracompact lightcycler nano. This lot comes with everything shown in the photos, to include. The lightcycler 480 software allows the user to easily alternate between screens e. Based on a comprehensive improvement of the peltierbased technology incorporated in a silver block, the instrument provides extraordinary welltowell temperature homogeneity and maximized interwell, intercycle reproducibility.
In preparation for real time polymerase chain reaction applications, small segments of dna or rna are isolated and placed in pcr plates. Most differences are obviously in the application software, especially in the way of. Lightcycler 480 genetic diversity centre eth zurich. Roche diagnostics 04729692001 lightcycler 480 multiwell plate 96, white 5 x 10 plates includes sealing foils catalog no.
The main switch is located at the back of the instrument. Lightcycler 480 relative quantification software 1 software package 04 727 851 001 lightcycler 480 multiwell plate 96 5 x 10 plates 04 729 692 001 lightcycler 480 multiwell plate 384 5 x 10 plates 04 729 749 001 lightcycler 480 probes master 5 x 1 ml 2x conc. Roche lightcycler 480 ii real time pcr system for 96 well plates. Lightcycler 96 system performance data roche life science. Lightcycler 480 software analysis screen for absolute quantification. I am going to design the probe for performingtaqman technology, how can i optimize the probes. Turn on the computer linked to the instrument and log into window xp. Has anybody used the light cycler 480 system by roche. The amplification of a viral target in twofold dilutions ranging from 1x10 2 to 1x10 5 copies per reaction is shown. The versatile, platebased lightcycler 96 system helps you achieve fast, highly sensitive, and reproducible results for qualitative and quantitative detection of nucleic acids and snp analysis compact and easy to use, the lightcycler 96 system primary is the ideal companion for applications including gene expression analysis, gene detection, mutation detection, methylation analysis. Work economically, adapting workflows to your format and throughput needs. Connection via network or usb stick, remote data analysis via email. Find safety data sheets, certificates of analysis and method sheets instructions for use in the roche support portal elabdoc in case of questions please contact your local custombiotech representative. Intuitive touchscreen and powerful analysis modules.
This lightcyler 480 instrument quick guide was created by the roche diagnostics corporation commercial education department. What if you had the power to get your qpcr data fast, reliably and with less effort. By using an internal control ic with the target in the same or a different well, inhibition of the pcr reaction e. The new lightcycler nano instrument is a 32well format realtime pcr cycler with state of the art functionality and easytouse software. Comparison of the taqman and lightcycler systems in. System component 1 lightcycler instrument sample carousel for o 1. Roche lightcycler ii laboratory thermal pcr system. Accurate results expected from a lightcycler system even with a gradient. The lightcycler 480 realtime pcr system roche applied science brings you one more breakthrough in the realtime pcr analysis of gene expression and genetic variation roche applied sciences cuttingedge gene analysis technology is now available in a highthroughput, microwell platebased cycler format 96 or 384well. Use the upper right quick search via catalog number or product name. Light cycler will saturate the detector somewhere around 500nm. We tested the same sample three times for both 96 and 384platforms to evaluate reproducibility of the cyp2b6 assay, and the results invariably overlapped with the two instruments. Contact your roche representative for further information.
Benefit from a cuttingedge, lowmass thermal block design that offers. The patented twocomponent microplate design provides superior stability and flatness, allowing precise. Roche launches new software updates for its lightcycler 96. Make sure the correct thermal block cycler 96well for f plate or 384well block format for g plate is in place. Iso certificate roche custombiotech in penzberg, germany. Realtime ready assays are available as single assays for the selected target or preplated on lightcycler 480 multiwell plates 96 or 384. Roche roche signs exclusive agreement with itis life. Discover this compact and smart new member of the roche lightcycler realtime pcr system. You can now create experimental protocols more quickly by using templates and macros.
Lightcycler 96 realtime pcr system from roche life science duration. The software is compatible with the regulations for electronic records subpart b. Direct questions or concerns regarding the contents of this document to. It offers fast and accurate analysis that impresses with its intuitive and userfriendly software. Available realtime rtpcr cycler and kinetic pcr machines. The system features the lightcycler 480 instrument, a versatile, platebased realtime pcr device.
Hardshell plates are specifically designed to withstand the stresses of heat sealing, thermal cycling, and robotic handling. The lightcycler 96 systems ergonomic design features a. The lightcycler 96 is a realtime pcr cycler for medium sample throughput. It enables the user to monitor the amplification of the pcr product simultaneously in realtime allowing the accurate quantification of target sequences. The user can also choose which samples to include in the display and. New software releases and user guides for the lightcycler 96 instrument are avail able in the. Supported by a broad range of software tools, realtime pcr based analysis can be performed in 32 capillaries, interchangeable 96384well plates or using the novel, unique 1536well format. Oligo probe 1 is labeled at the 3 end with a donor reporter and oligo probe 2 is labeled at its 5 end with one of a few available acceptor reporters. Complementing roches lightcycler 480 and lightcycler 1536 realtime pcr systems, it offers a convenient solution for labs currently sharing time on another labs realtime pcr instrument. Realtime ready custom panels create your specific gene expression panel with realtime ready assays of your choice on lightcycler 480 multiwell plates 96 or 384, using the realtime ready configurator. The 96well block for the f plate format or the 384well block for g plate format.
How to generate qpcr standard curve in lightcycler 96. Eurofins dna quantification and snp detection on gold standard platforms. With a total of 6 detection channels, this compact cycler offers the highest flexibility and allows single color and multiplex testing. Speed time to results with advanced yet easytouse software. The cycler has 4 detection channels, which detect hydrolysis 5nuclease taqman probes either as single color or multiplex samples.
A lightcycler probe system consists of a pair of singlestranded fluorescentlabeled oligonucleotides. Hardshell 96well 480 pcr plates are optimized to work on the roche lightcycler 480 realtime pcr system. Roche applied science lightcycler 480 realtime pcr system. Lightcycler 480 multiwell plate 96, white 5 x 10 plates includes sealing foils catalog no. Service manual light cycler please note that this is only a. Roche lightcycler 480ii384 pcr w thermocycler 96 s 2. Roche launches new software updates for its lightcycler 96 and lightcycler nano pcr systems. System component 2 lightcycler capillaries 96 capillaries and stoppersbox1 32 lightcycler centrifuge adapters in an aluminum cooling block1 1 lightcycler operators manual 1 lightcycler software package1 cable to connect lightcycler instrument to the computer power cord german power cord u. New innovative lightcycler 96 application and instrument software creates a simple. The lightcycler 480 can be reserved via the electronic reservations system.
Supported by a broad range of software tools, realtime pcr based analysis can be performed in 32 capillaries, interchangeable 96 384well plates or using the novel, unique 1536well format. In contrast, the biorad cfx is our goto machine for most 96 well applications. The lightcycler 96 software s qualitative detection module allows for reliable analysis of target genes and controls, using two or even more colors. I am using the lightcycler 480 sybr green i master kit from roche, but it is brand new. Software will calculate the standard curve slope, efficiency, error, r2, and yintercept relquant if not already done open experimental file on lightcycler 96 user software. Service manual light cycler please note that this is only. An ideal combination of accuracy, temperature homogeneity, and reproducibility, matched with powerful software so intuitive that it is accessible to any user. Analysis software is supplied to permit the analysis of lightcycler 96. An ideal combination of accuracy, temperature homogeneity, and reproducibility, matched with powerful software so intuitive that it is. Roche molecular biochemicals lightcycler operators manual. If you have already disposed of the packaging, you can request it from roche.
Roche life science realtime ready overview lightcycler. Depending on the experimental design, the system can even help you select and. With common well matrixes and high or low profile designs that are compatible with thermal cyclers, the pcr plates facilitate. The lightcycler 480 system is a compact and versatile benchtop instrument that can hold multiwell plates for either.
An ideal combination of accuracy, temperature homogeneity, and reproducibility, matched with powerful software so. The lightcycler 96 system delivers data that makes you a. Roche today announced the global launch of new software updates, including advanced high resolution melting analysis and qualitative gene detection modules for the light cycler 96 and new support features for the lightcycler nano system. These are low profile microplates compatible with many thermal cyclers, but especially suitable for the roche lightcycler 480. Roche lightcycler 480ii384 pcr w thermocycler 96s 2. With common well matrixes and high or low profile designs that are compatible with thermal cyclers, the pcr. Warranty information on warranty conditions are specified in the sales contract. For your information, we are using a roche lightcycler 480 and as a dye we use eva green without rox. However, the software or at least the version that we have is not. To import your sample names into the lightcycler software use the plate maker file. Roche lightcycler 96 biotecon diagnostics biotecon.
The result of more than a decade of roche experience developing highperformance qpcr systems is now accessible for any research lab. For todays academic researcher labs, the new roche lightcycler 96 system is the optimal midsize, midthroughput realtime pcr solution that conveniently delivers the highest standards expected from a lightcycler system. Nov 21, 2019 for your information, we are using a roche lightcycler 480 and as a dye we use eva green without rox. The lightcycler 480 system is a highperformance, flexible throughput qpcr platform interchangeable 96 or 384well blocks that provides a platebased, highly adaptable and versatile realtime pcr system for gene expression analysis, snp genotyping, and mutation scanning via high resolution melting hrm. Roche light cycler 48096 pcr thermal cycler 96 well andbio. Compact and easy to use, the lightcycler 96 system primary is the ideal companion for applications including gene expression analysis, gene detection, mutation detection, methylation analysis, mirna research, and relative quantification of target genes. I prepared the twostep cycling experiment with the following conditions. Roche lightcycler 480 garvan institute of medical research. I have a problem with the analysis in lightcycler 480 software. The lightcycler instrument of roche molecular biochemicals, mannheim, ge rmany, is a thermocycler for the rapid analysis of pcr applications.
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